Q (haiku): show me all KRAS mutations in colorectal cancer that are not at position 12

▶ read_guide
{
 "uri": "cbioportal://mutation-frequency-guide"
}

▶ search_oncotree
{
 "search_term": "colorectal"
}

◀ result
# Mutation Frequency Analysis Guide

## IMPORTANT: Reporting Mutation Frequencies
- **ALWAYS report frequencies as percentages**, not raw counts: `frequency = (altered_samples / total_profiled_samples) × 100`
- For quick frequency lookups, **prefer the TCGA Pan-Cancer Atlas study first**, then offer to expand to other studies
- When reporting across multiple studies, show **ranges** (e.g., "TP53 is mutated in 30–60% of samples") rather than a single average
- **NEVER** sum mutation events across studies to compute an aggregate frequency — this can exceed 100% due to double-counting
- Warn users that samples may overlap across cohorts (e.g., MSK studies may share patients)
- **Choose and state the counting unit**: use patient-level frequencies for prevalence/rate questions unless the user explicitly asks for samples; use sample-level frequencies when the user asks about samples.
- **For "across cancer types" questions**, jump to the [Cross-Cancer-Type Mutation Frequency](#cross-cancer-type-mutation-frequency) section below — there is one correct recipe and several common wrong ones.

## Counting Unit: Samples vs Patients

Before answering any mutation count or frequency question, decide whether the unit is samples or patients and state that choice in the answer.

| User wording | Counting unit |
|--------------|---------------|
| "prevalence", "rate", "fraction of patients", "patients with", "how common is" | Patient-level: `COUNT(DISTINCT patient_unique_id)` |
| "samples", "specimens", "biopsies", sample-level cohort composition | Sample-level: `COUNT(DISTINCT sample_unique_id)` |
| Ambiguous | Ask, or default to patient-level for prevalence/rate language and say so |

### Cross-study sample-count caveat

When an answer touches more than one study and reports a sample count, prepend a one-line caveat:

> Sample IDs are unique within cBioPortal study prefixes, not guaranteed biological-sample identifiers across studies; overlapping cohorts can count the same patient/sample more than once.

Prefer one of these safer approaches:

- Use a shipped `cancer_study_query_preferences` cohort such as `pan_cancer_tcga` or `all_studies_non_redundant`.
- Restrict to one named study.
- Aggregate by `patient_unique_id` when the biological question is patient prevalence.

## STOP rule: a frequency above 100% means your query is wrong

If your query returns a frequency over 100%, **do not try to debug or explain the data inconsistency to the user**. The cause is always one of these query bugs:

- Summing mutation events instead of `COUNT(DISTINCT sample_unique_id)` for the numerator
- Using a study-wide sample count as the denominator instead of the gene-specific profiled count
- Cross-study aggregation where the same biological sample appears under multiple `sample_unique_id` values (e.g., MSK-IMPACT and MSK-CHORD share patients)
- **Joining the profiled CTE through `gene_panel` / `gene_panel_list` without a WES branch.** `gene_panel_id = 'WES'` is *not* a row in … (36467 more chars)

▶ list_studies
{
 "search": "colorectal",
 "limit": 20
}

◀ result
{"result":[{"code":"COADREAD","name":"Colorectal Adenocarcinoma","score":60,"mainType":"Colorectal Cancer","tissue":"Bowel","hierarchy":"BOWEL > COADREAD"},{"code":"CAIS","name":"Colon Adenocarcinoma In Situ","score":45,"mainType":"Colorectal Cancer","tissue":"Bowel","hierarchy":"BOWEL > COADREAD > CAIS"},{"code":"CMC","name":"Medullary Carcinoma of the Colon","score":45,"mainType":"Colorectal Cancer","tissue":"Bowel","hierarchy":"BOWEL > CMC"},{"code":"COAD","name":"Colon Adenocarcinoma","score":45,"mainType":"Colorectal Cancer","tissue":"Bowel","hierarchy":"BOWEL > COADREAD > COAD"},{"code":"MACR","name":"Mucinous Adenocarcinoma of the Colon and Rectum","score":45,"mainType":"Colorectal Cancer","tissue":"Bowel","hierarchy":"BOWEL > COADREAD > MACR"},{"code":"READ","name":"Rectal Adenocarcinoma","score":45,"mainType":"Colorectal Cancer","tissue":"Bowel","hierarchy":"BOWEL > COADREAD > READ"},{"code":"SRCCR","name":"Signet Ring Cell Adenocarcinoma of the Colon and Rectum","score":45,"mainType":"Colorectal Cancer","tissue":"Bowel","hierarchy":"BOWEL > COADREAD > SRCCR"}]}

◀ result
{"result":[{"cancer_study_identifier":"msk_met_2021","name":"MSK MetTropism (MSK, Cell 2021)","type_of_cancer_id":"mixed","sample_count":25775,"has_guide":false,"url":"https://www.cbioportal.org/study/summary?id=msk_met_2021"},{"cancer_study_identifier":"crc_msk_2026","name":"Colorectal Adenocarcinoma (MSK, 2026)","type_of_cancer_id":"read","sample_count":7237,"has_guide":false,"url":"https://www.cbioportal.org/study/summary?id=crc_msk_2026"},{"cancer_study_identifier":"crc_eo_2020","name":"Colorectal Cancer (MSK, JNCI 2021)","type_of_cancer_id":"bowel","sample_count":1516,"has_guide":false,"url":"https://www.cbioportal.org/study/summary?id=crc_eo_2020"},{"cancer_study_identifier":"crc_msk_2017","name":"Metastatic Colorectal Cancer (MSK, Cancer Cell 2018)","type_of_cancer_id":"coadread","sample_count":1134,"has_guide":false,"url":"https://www.cbioportal.org/study/summary?id=crc_msk_2017"},{"cancer_study_identifier":"crc_sysucc_2022","name":"Colorectal Cancer- ChangKang Project (SYSUCC, Nat Commun 2022)","type_of_cancer_id":"coadread","sample_count":1015,"has_guide":false,"url":"https://www.cbioportal.org/study/summary?id=crc_sysucc_2022"},{"cancer_study_identifier":"coadread_tcga","name":"Colorectal Adenocarcinoma (TCGA, Firehose Legacy)","type_of_cancer_id":"coadread","sample_count":640,"has_guide":false,"url":"https://www.cbioportal.org/study/summary?id=coadread_tcga"},{"cancer_study_identifier":"coadread_dfci_2016","name":"Colorectal Adenocarcinoma (DFCI, Cell Reports 2016)","type_of_cancer_id":"coadread","sample_count":619,"has_guide":false,"url":"https://www.cbioportal.org/study/summary?id=coadread_dfci_2016"},{"cancer_study_identifier":"coadread_tcga_pan_can_atlas_2018","name":"Colorectal Adenocarcinoma (TCGA, PanCancer Atlas)","type_of_cancer_id":"coadread","sample_count":594,"has_guide":true,"url":"https://www.cbioportal.org/study/summary?id=coadread_tcga_pan_can_atlas_2018"},{"cancer_study_identifier":"crc_apc_impact_2020","name":"Colorectal Cancer (MSK, Gastroenterology 2020)","type_of_cancer_id":"coadread","sample_count":471,"has_guide":false,"url":"https://www.cbioportal.org/study/summary?id=crc_apc_impact_2020"},{"cancer_study_identifier":"coadread_tcga_pub","name":"Colorectal Adenocarcinoma (TCGA, Nature 2012)","type_of_cancer_id":"coadread","sample_count":276,"has_guide":false,"url":"https://www.cbioportal.org/study/summary?id=coadread_tcga_pub"},{"cancer_study_identifier":"bowel_colitis_msk_2022","name":"Colorectal Adenocarcinoma (MSK, Nat Commun 2022)","type_of_cancer_id":"bowel","sample_count":179,"has_guide":false,"url":"https://www.cbioportal.org/study/summary?id=bowel_colitis_msk_2022"},{"cancer_study_identifier":"coadread_cass_2020","name":"Colorectal Cancer (CAS Shanghai, Cancer Cell 2020)","type_of_cancer_id":"coadread","sample_count":146,"has_guide":false,"url":"https://www.cbioportal.org/study/summary?id=coadread_cass_2020"},{"cancer_study_identifier":"coadread_mskcc","name":"Colorectal Adenocarcinoma Triplets (MSK, Geno … (1887 more chars)

▶ clickhouse_list_table_columns
{
 "table": "genomic_event_derived"
}

◀ result
{"columns":[{"name":"sample_unique_id","type":"String"},{"name":"hugo_gene_symbol","type":"String","comment":"HUGO gene symbol (e.g., TP53, KRAS, BRAF). Use for gene-specific queries."},{"name":"entrez_gene_id","type":"Int32"},{"name":"gene_panel_stable_id","type":"LowCardinality(String)"},{"name":"cancer_study_identifier","type":"LowCardinality(String)"},{"name":"genetic_profile_stable_id","type":"LowCardinality(String)"},{"name":"variant_type","type":"LowCardinality(String)","comment":"Type of genomic event: \"mutation\" for SNVs/indels, \"cna\" for copy number alterations, \"structural_variant\" for SVs. Always filter by this."},{"name":"mutation_variant","type":"String","comment":"Protein change notation (e.g., p.V600E, p.R175H). Use for specific variant queries. \"NA\" for non-mutation events."},{"name":"mutation_type","type":"LowCardinality(String)"},{"name":"mutation_status","type":"LowCardinality(String)","comment":"For mutations: Somatic, Germline, UNKNOWN, or UNCALLED. Filter mutation_status != \"UNCALLED\" to exclude uncertain calls. Include all other statuses."},{"name":"driver_filter","type":"LowCardinality(String)"},{"name":"driver_filter_annotation","type":"String"},{"name":"driver_tiers_filter","type":"LowCardinality(String)"},{"name":"driver_tiers_filter_annotation","type":"String"},{"name":"cna_alteration","type":"Nullable(Int8)","comment":"Copy number alteration: only 2 = amplification (AMP) and -2 = deep deletion (HOMDEL) are stored. Shallow deletion (-1), diploid (0) and gain (1) are not in this table; query genetic_alteration_derived WHERE profile_type = 'gistic' (alteration_value is a String, e.g. '-1'). NULL for non-CNA events."},{"name":"cna_cytoband","type":"String"},{"name":"sv_event_info","type":"String"},{"name":"patient_unique_id","type":"String"},{"name":"off_panel","type":"Bool","comment":"Boolean: 1 = mutation outside gene panel coverage (off-panel), 0 = within panel (on-panel). Filter off_panel = 0 for reliable frequency calculations."}]}

▶ clickhouse_run_select_query
{
 "query": "
SELECT
    mutation_variant,
    mutation_type,
    COUNT(DISTINCT sample_unique_id) AS sample_count,
    COUNT(*) AS total_events
FROM genomic_event_derived
WHERE cancer_study_identifier = 'coadread_tcga_pan_can_atlas_2018'
  AND hugo_gene_symbol = 'KRAS'
  AND variant_type = 'mutation'
  AND mutation_status != 'UNCALLED'
  AND off_panel = 0
  AND mutation_variant NOT LIKE '%12%'  -- Exclude position 12
ORDER BY sample_count DESC
",
 "max_rows": 100
}

◀ result
{"error_message":"Query execution failed: HTTPDriver for https://dl96orhu96.us-east-1.aws.clickhouse.cloud:8443 received ClickHouse error code 215\n Code: 215. DB::Exception: Column 'cbioportal_public_librechat_blue.genomic_event_derived.mutation_variant' is not under aggregate function and not in GROUP BY keys. In query SELECT mutation_variant, mutation_type, COUNTDistinct(sample_unique_id) AS sample_count, COUNT(*) AS total_events FROM genomic_event_derived WHERE (cancer_study_identifier = 'coadread_tcga_pan_can_atlas_2018') AND (hugo_gene_symbol = 'KRAS') AND (variant_type = 'mutation') AND (mutation_status != 'UNCALLED') AND (off_panel = 0) AND (mutation_variant NOT LIKE '%12%') ORDER BY sample_count DESC. (NOT_AN_AGGREGATE) (version 26.4.1.2359 (official build))\n"}

▶ clickhouse_run_select_query
{
 "query": "
SELECT
    mutation_variant,
    mutation_type,
    COUNT(DISTINCT sample_unique_id) AS sample_count,
    COUNT(*) AS total_events
FROM genomic_event_derived
WHERE cancer_study_identifier = 'coadread_tcga_pan_can_atlas_2018'
  AND hugo_gene_symbol = 'KRAS'
  AND variant_type = 'mutation'
  AND mutation_status != 'UNCALLED'
  AND off_panel = 0
  AND mutation_variant NOT LIKE '%12%'  -- Exclude position 12
GROUP BY mutation_variant, mutation_type
ORDER BY sample_count DESC
",
 "max_rows": 100
}

◀ result
{"rows":[{"mutation_variant":"G13D","mutation_type":"Missense_Mutation","sample_count":37,"total_events":37},{"mutation_variant":"A146T","mutation_type":"Missense_Mutation","sample_count":16,"total_events":16},{"mutation_variant":"K117N","mutation_type":"Missense_Mutation","sample_count":4,"total_events":4},{"mutation_variant":"Q61H","mutation_type":"Missense_Mutation","sample_count":4,"total_events":4},{"mutation_variant":"Q61K","mutation_type":"Missense_Mutation","sample_count":3,"total_events":3},{"mutation_variant":"Q61R","mutation_type":"Missense_Mutation","sample_count":2,"total_events":2},{"mutation_variant":"G13C","mutation_type":"Missense_Mutation","sample_count":2,"total_events":2},{"mutation_variant":"Q61E","mutation_type":"Missense_Mutation","sample_count":1,"total_events":1},{"mutation_variant":"Q22K","mutation_type":"Missense_Mutation","sample_count":1,"total_events":1},{"mutation_variant":"Y71C","mutation_type":"Missense_Mutation","sample_count":1,"total_events":1},{"mutation_variant":"R68S","mutation_type":"Missense_Mutation","sample_count":1,"total_events":1},{"mutation_variant":"A59T","mutation_type":"Missense_Mutation","sample_count":1,"total_events":1},{"mutation_variant":"L19F","mutation_type":"Missense_Mutation","sample_count":1,"total_events":1},{"mutation_variant":"P34L","mutation_type":"Missense_Mutation","sample_count":1,"total_events":1},{"mutation_variant":"A146V","mutation_type":"Missense_Mutation","sample_count":1,"total_events":1},{"mutation_variant":"Q61P","mutation_type":"Missense_Mutation","sample_count":1,"total_events":1},{"mutation_variant":"A155D","mutation_type":"Missense_Mutation","sample_count":1,"total_events":1},{"mutation_variant":"Q61L","mutation_type":"Missense_Mutation","sample_count":1,"total_events":1},{"mutation_variant":"E98*","mutation_type":"Nonsense_Mutation","sample_count":1,"total_events":1}]}

▶ clickhouse_run_select_query
{
 "query": "
SELECT COUNT(DISTINCT sample_unique_id) AS kras_profiled_samples
FROM sample_to_gene_panel_derived
WHERE cancer_study_identifier = 'coadread_tcga_pan_can_atlas_2018'
  AND hugo_gene_symbol = 'KRAS'
  AND alteration_type = 'MUTATION_EXTENDED'
",
 "max_rows": 10
}

◀ result
{"error_message":"Query execution failed: HTTPDriver for https://dl96orhu96.us-east-1.aws.clickhouse.cloud:8443 received ClickHouse error code 47\n Code: 47. DB::Exception: Unknown expression or function identifier `hugo_gene_symbol` in scope SELECT COUNTDistinct(sample_unique_id) AS kras_profiled_samples FROM sample_to_gene_panel_derived WHERE (cancer_study_identifier = 'coadread_tcga_pan_can_atlas_2018') AND (hugo_gene_symbol = 'KRAS') AND (alteration_type = 'MUTATION_EXTENDED'). (UNKNOWN_IDENTIFIER) (version 26.4.1.2359 (official build))\n"}

▶ clickhouse_list_table_columns
{
 "table": "mutation_panel_gene_coverage"
}

◀ result
{"columns":[{"name":"sample_unique_id","type":"String"},{"name":"cancer_study_identifier","type":"LowCardinality(String)"},{"name":"hugo_gene_symbol","type":"String"},{"name":"gene_panel_id","type":"LowCardinality(String)"}]}

▶ clickhouse_run_select_query
{
 "query": "
SELECT COUNT(DISTINCT sample_unique_id) AS kras_profiled_samples
FROM mutation_panel_gene_coverage
WHERE cancer_study_identifier = 'coadread_tcga_pan_can_atlas_2018'
  AND hugo_gene_symbol = 'KRAS'

UNION ALL

SELECT COUNT(DISTINCT sample_unique_id) AS kras_profiled_samples
FROM mutation_wes_coverage
WHERE cancer_study_identifier = 'coadread_tcga_pan_can_atlas_2018'
",
 "max_rows": 10
}

◀ result
{"rows":[{"kras_profiled_samples":534},{"kras_profiled_samples":0}]}

▶ resolve_and_route
{
 "studyIds": [
  "coadread_tcga_pan_can_atlas_2018"
 ]
}

◀ result
{"success":true,"message":"Found 1 study. Use the metadata to call the appropriate navigation tool(s).","data":{"totalCount":1,"studiesWithMetadata":[{"studyId":"coadread_tcga_pan_can_atlas_2018","name":"Colorectal Adenocarcinoma (TCGA, PanCancer Atlas)","sampleCount":594,"studyViewUrl":"https://www.cbioportal.org/study?id=coadread_tcga_pan_can_atlas_2018","metadata":{"clinicalAttributeIds":["AGE","AJCC_PATHOLOGIC_TUMOR_STAGE","AJCC_STAGING_EDITION","ANEUPLOIDY_SCORE","BUFFA_HYPOXIA_SCORE","CANCER_TYPE","CANCER_TYPE_ACRONYM","CANCER_TYPE_DETAILED","DAYS_LAST_FOLLOWUP","DAYS_TO_BIRTH","DAYS_TO_INITIAL_PATHOLOGIC_DIAGNOSIS","DFS_MONTHS","DFS_STATUS","DSS_MONTHS","DSS_STATUS","ETHNICITY","FORM_COMPLETION_DATE","FRACTION_GENOME_ALTERED","GENETIC_ANCESTRY_LABEL","GRADE","HISTORY_NEOADJUVANT_TRTYN","ICD_10","ICD_O_3_HISTOLOGY","ICD_O_3_SITE","INFORMED_CONSENT_VERIFIED","IN_PANCANPATHWAYS_FREEZE","MSI_SCORE_MANTIS","MSI_SENSOR_SCORE","MUTATION_COUNT","NEW_TUMOR_EVENT_AFTER_INITIAL_TREATMENT","ONCOTREE_CODE","OS_MONTHS","OS_STATUS","OTHER_PATIENT_ID","PATH_M_STAGE","PATH_N_STAGE","PATH_T_STAGE","PERSON_NEOPLASM_CANCER_STATUS","PFS_MONTHS","PFS_STATUS","PRIMARY_LYMPH_NODE_PRESENTATION_ASSESSMENT","PRIOR_DX","RACE","RADIATION_THERAPY","RAGNUM_HYPOXIA_SCORE","SAMPLE_COUNT","SAMPLE_TYPE","SEX","SOMATIC_STATUS","SUBTYPE","TBL_SCORE","TISSUE_PROSPECTIVE_COLLECTION_INDICATOR","TISSUE_RETROSPECTIVE_COLLECTION_INDICATOR","TISSUE_SOURCE_SITE","TISSUE_SOURCE_SITE_CODE","TMB_NONSYNONYMOUS","TUMOR_TISSUE_SITE","TUMOR_TYPE","WEIGHT","WINTER_HYPOXIA_SCORE"],"molecularProfileIds":["coadread_tcga_pan_can_atlas_2018_gistic","coadread_tcga_pan_can_atlas_2018_log2CNA","coadread_tcga_pan_can_atlas_2018_mutations","coadread_tcga_pan_can_atlas_2018_protein_quantification","coadread_tcga_pan_can_atlas_2018_protein_quantification_zscores","coadread_tcga_pan_can_atlas_2018_rna_seq_v2_mrna","coadread_tcga_pan_can_atlas_2018_rna_seq_v2_mrna_median_Zscores","coadread_tcga_pan_can_atlas_2018_rna_seq_v2_mrna_median_all_sample_Zscores","coadread_tcga_pan_can_atlas_2018_rna_seq_v2_mrna_median_all_sample_ref_normal_Zscores","coadread_tcga_pan_can_atlas_2018_rppa","coadread_tcga_pan_can_atlas_2018_rppa_Zscores","coadread_tcga_pan_can_atlas_2018_structural_variants"],"genericAssayProfiles":["coadread_tcga_pan_can_atlas_2018_armlevel_cna","coadread_tcga_pan_can_atlas_2018_genetic_ancestry","coadread_tcga_pan_can_atlas_2018_methylation_hm27_hm450_merge","coadread_tcga_pan_can_atlas_2018_methylation_hm450"],"heatmapProfileIds":["coadread_tcga_pan_can_atlas_2018_protein_quantification_zscores","coadread_tcga_pan_can_atlas_2018_rna_seq_v2_mrna_median_Zscores","coadread_tcga_pan_can_atlas_2018_rna_seq_v2_mrna_median_all_sample_Zscores","coadread_tcga_pan_can_atlas_2018_rna_seq_v2_mrna_median_all_sample_ref_normal_Zscores","coadread_tcga_pan_can_atlas_2018_rppa_Zscores"],"availableComparisonTabs":["overlap","clinical","survival","alterations","mutations","mrna","protein","generic_assay_armlevel_c … (70 more chars)

▶ navigate_to_results_view
{
 "studyIds": [
  "coadread_tcga_pan_can_atlas_2018"
 ],
 "genes": [
  "KRAS: MUT != G12*"
 ],
 "tab": "mutations"
}

◀ result
{"success":true,"message":"Navigating to https://www.cbioportal.org/results/mutations?cancer_study_list=coadread_tcga_pan_can_atlas_2018&gene_list=KRAS%3A+MUT+%21%3D+G12*&case_set_id=coadread_tcga_pan_can_atlas_2018_all","url":"https://www.cbioportal.org/results/mutations?cancer_study_list=coadread_tcga_pan_can_atlas_2018&gene_list=KRAS%3A+MUT+%21%3D+G12*&case_set_id=coadread_tcga_pan_can_atlas_2018_all","data":{"studyIds":["coadread_tcga_pan_can_atlas_2018"],"studies":[{"studyId":"coadread_tcga_pan_can_atlas_2018","name":"Colorectal Adenocarcinoma (TCGA, PanCancer Atlas)","sampleCount":594}],"genes":["KRAS"],"caseSetId":"coadread_tcga_pan_can_atlas_2018_all","pageDescription":"Detailed mutation table with lollipop plot showing mutation positions, types, and frequencies for queried genes."}}

═ answer (success)
Perfect! Here are all KRAS mutations in colorectal adenocarcinoma (TCGA PanCancer Atlas) that are **not at position 12**:

## KRAS Mutations Excluding Position 12

**Study:** Colorectal Adenocarcinoma (TCGA, PanCancer Atlas) — 594 samples, 534 KRAS-profiled

| Variant | Mutation Type | Samples | Frequency |
|---------|---------------|---------|-----------|
| **G13D** | Missense | 37 | 6.9% |
| **A146T** | Missense | 16 | 3.0% |
| **K117N** | Missense | 4 | 0.7% |
| **Q61H** | Missense | 4 | 0.7% |
| **Q61K** | Missense | 3 | 0.6% |
| **Q61R** | Missense | 2 | 0.4% |
| **G13C** | Missense | 2 | 0.4% |
| **Q61E** | Missense | 1 | 0.2% |
| **Q22K** | Missense | 1 | 0.2% |
| **Y71C** | Missense | 1 | 0.2% |
| **R68S** | Missense | 1 | 0.2% |
| **A59T** | Missense | 1 | 0.2% |
| **L19F** | Missense | 1 | 0.2% |
| **P34L** | Missense | 1 | 0.2% |
| **A146V** | Missense | 1 | 0.2% |
| **Q61P** | Missense | 1 | 0.2% |
| **A155D** | Missense | 1 | 0.2% |
| **Q61L** | Missense | 1 | 0.2% |
| **E98\*** | Nonsense | 1 | 0.2% |

**Total non-position-12 KRAS mutations: 79 samples (14.8% of profiled)**

The vast majority of non-position-12 KRAS mutations cluster at **position 13 (G13D/G13C)** and **codon 61 (Q61H/K/R/E/P/L)**, both known oncogenic hotspots. Position 146 (A146T/V) is less common but recurrent.

[**View all KRAS mutations in the Mutations tab →**](https://www.cbioportal.org/results/mutations?cancer_study_list=coadread_tcga_pan_can_atlas_2018&gene_list=KRAS%3A+MUT+%21%3D+G12*&case_set_id=coadread_tcga_pan_can_atlas_2018_all)